1211 Iris florentina L. extract: Inhibitor and inducer of epidermal melanogenesis

Abnormal changes in skin pigmentation cause considerable cosmetic problems and affect the quality of life. Skin is mainly determined by a pigment called melanin, regulation melanin synthesis incredibly intricate, yet it has become an important strategy global industry leading to development technologies that mitigate unwanted conditions. In this study, we examined effect extract from root rhizome Iris florentina L.(orris root) on capacity melanogenesis. Bioassay-directed fractionation (BDF) identified phytochemicals interest collecting fractions LC-MS effluent into 96 well plates testing for melanogenesis activity. Fractions 41-43 yielded higher content germanaism B was subsequently as stimulatory agent; 90-93 decreased compounds iriflorental/iripallidal were inhibitory agents. Active purified further analyzed using B16 cells human primary melanocytes. Germanaism significantly induced production both models whereas iriflorental iripallidal demonstrated depigmenting effect. We then prepared ethanolic supercritical carbon dioxide (SC-CO2) extracts I. root/rhizome determine if could generate two separate with opposite effects The SC-CO2 evaluated 3D equivalent tissues synthesis, SC-CO2extract inhibited production. report first time germanica contains active components having melanogenesis, making possible prevent or ameliorate hypopigmentation hyperpigmentation conditions one botanical source.